DUOX BIOTECHNOLOGIES (Transgen LLC) is an R&D partner for biomedical teams that need fast, reproducible research tools: cellular models, stable cell lines, and genetic constructs. The company takes on the labor‑intensive “engineering” part of a project - from vector assembly to precise genome editing - so your lab can stay focused on hypotheses, data, and publications.
Achievements and R&D strengths
The company has built a portfolio of applied technologies at the intersection of molecular biology and cell engineering and offers solutions for different research strategies:
1) Cell engineering and model generation
Transgenesis of human and animal cells.
Gene knockout, introduction of point mutations (SNS), and integration of sequences into the genome: via Sleeping Beauty and PiggyBac,
into Safe Harbor loci (Rosa, Hipp11),
into any loci via homologous recombination.
Inducible systems: LoxP/Cre‑based,
doxycycline‑inducible systems.
Reporter systems in human and animal cells using fluorescent proteins.
Producer cell lines for expression of target proteins.
2) Genetic construct and vector assembly
Vector assembly according to the customer’s map/design.
Vectors for: production of AAV of various serotypes,
prokaryotic expression (inducible and constitutive),
eukaryotic expression (inducible and constitutive),
cloning vectors,
cloning of repeated sequences (as a separate complex category of tasks).
How this helps your project
DUOX BIOTECHNOLOGIES operates as an “R&D builder”: you can order a standard task (e.g., a knockout, a reporter, or stable integration), or assemble a unique pipeline tailored to your hypothesis - from vector design to a cell system ready for screening, validation, and further platform development. Pricing is calculated individually, which is convenient for non‑standard projects and combined technical requirements.
Achievements and R&D strengths
The company has built a portfolio of applied technologies at the intersection of molecular biology and cell engineering and offers solutions for different research strategies:
- Controlled transgene integration into Safe Harbor loci (e.g., Rosa26 and Hipp11) for applications where predictable expression, minimization of positional effects, and controlled copy number are critical. This is especially important for reporter lines, stable expression, and comparative experiments.
- High‑efficiency transgenesis using Sleeping Beauty - a fast route to generating transgenic cells with integration of DNA fragments up to 15 kb, when speed and efficiency matter more than strict control over insertion site/copy number.
- Cell immortalization via telomerase, with selection of the optimal option based on the origin of the primary cell line and the ability to implement both integrative and non‑integrative approaches. This enables conversion of primary cells into a convenient format for long experimental series.
- Recognition of technological maturity: the company has been granted the status of a small technology company in the registry of the Ministry of Economic Development of the Russian Federation - a marker of systematic R&D expertise and a robust technology base.
1) Cell engineering and model generation
Transgenesis of human and animal cells.
Gene knockout, introduction of point mutations (SNS), and integration of sequences into the genome: via Sleeping Beauty and PiggyBac,
into Safe Harbor loci (Rosa, Hipp11),
into any loci via homologous recombination.
Inducible systems: LoxP/Cre‑based,
doxycycline‑inducible systems.
Reporter systems in human and animal cells using fluorescent proteins.
Producer cell lines for expression of target proteins.
2) Genetic construct and vector assembly
Vector assembly according to the customer’s map/design.
Vectors for: production of AAV of various serotypes,
prokaryotic expression (inducible and constitutive),
eukaryotic expression (inducible and constitutive),
cloning vectors,
cloning of repeated sequences (as a separate complex category of tasks).
How this helps your project
DUOX BIOTECHNOLOGIES operates as an “R&D builder”: you can order a standard task (e.g., a knockout, a reporter, or stable integration), or assemble a unique pipeline tailored to your hypothesis - from vector design to a cell system ready for screening, validation, and further platform development. Pricing is calculated individually, which is convenient for non‑standard projects and combined technical requirements.
DUOX BIOTECHNOLOGIES (Transgen LLC) is an R&D partner for biomedical teams that need fast, reproducible research tools: cellular models, stable cell lines, and genetic constructs. The company takes on the labor‑intensive “engineering” part of a project - from vector assembly to precise genome editing - so your lab can stay focused on hypotheses, data, and publications.
Achievements and R&D strengths
The company has built a portfolio of applied technologies at the intersection of molecular biology and cell engineering and offers solutions for different research strategies:
R&D services: what DUOX BIOTECHNOLOGIES does
1) Cell engineering and model generation
Transgenesis of human and animal cells.
Gene knockout, introduction of point mutations (SNS), and integration of sequences into the genome: via Sleeping Beauty and PiggyBac,
into Safe Harbor loci (Rosa, Hipp11),
into any loci via homologous recombination.
Inducible systems: LoxP/Cre‑based,
doxycycline‑inducible systems.
Reporter systems in human and animal cells using fluorescent proteins.
Producer cell lines for expression of target proteins.
2) Genetic construct and vector assembly
Vector assembly according to the customer’s map/design.
Vectors for: production of AAV of various serotypes,
prokaryotic expression (inducible and constitutive),
eukaryotic expression (inducible and constitutive),
cloning vectors,
cloning of repeated sequences (as a separate complex category of tasks).
How this helps your project
DUOX BIOTECHNOLOGIES operates as an “R&D builder”: you can order a standard task (e.g., a knockout, a reporter, or stable integration), or assemble a unique pipeline tailored to your hypothesis - from vector design to a cell system ready for screening, validation, and further platform development. Pricing is calculated individually, which is convenient for non‑standard projects and combined technical requirements.
Achievements and R&D strengths
The company has built a portfolio of applied technologies at the intersection of molecular biology and cell engineering and offers solutions for different research strategies:
- Controlled transgene integration into Safe Harbor loci (e.g., Rosa26 and Hipp11) for applications where predictable expression, minimization of positional effects, and controlled copy number are critical. This is especially important for reporter lines, stable expression, and comparative experiments.
- High‑efficiency transgenesis using Sleeping Beauty - a fast route to generating transgenic cells with integration of DNA fragments up to 15 kb, when speed and efficiency matter more than strict control over insertion site/copy number.
- Cell immortalization via telomerase, with selection of the optimal option based on the origin of the primary cell line and the ability to implement both integrative and non‑integrative approaches. This enables conversion of primary cells into a convenient format for long experimental series.
- Recognition of technological maturity: the company has been granted the status of a small technology company in the registry of the Ministry of Economic Development of the Russian Federation - a marker of systematic R&D expertise and a robust technology base.
R&D services: what DUOX BIOTECHNOLOGIES does
1) Cell engineering and model generation
Transgenesis of human and animal cells.
Gene knockout, introduction of point mutations (SNS), and integration of sequences into the genome: via Sleeping Beauty and PiggyBac,
into Safe Harbor loci (Rosa, Hipp11),
into any loci via homologous recombination.
Inducible systems: LoxP/Cre‑based,
doxycycline‑inducible systems.
Reporter systems in human and animal cells using fluorescent proteins.
Producer cell lines for expression of target proteins.
2) Genetic construct and vector assembly
Vector assembly according to the customer’s map/design.
Vectors for: production of AAV of various serotypes,
prokaryotic expression (inducible and constitutive),
eukaryotic expression (inducible and constitutive),
cloning vectors,
cloning of repeated sequences (as a separate complex category of tasks).
How this helps your project
DUOX BIOTECHNOLOGIES operates as an “R&D builder”: you can order a standard task (e.g., a knockout, a reporter, or stable integration), or assemble a unique pipeline tailored to your hypothesis - from vector design to a cell system ready for screening, validation, and further platform development. Pricing is calculated individually, which is convenient for non‑standard projects and combined technical requirements.